CoraLite® Plus 647 Anti-Human CD107a / LAMP1 Rabbit scFv Antibody

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• CoraLite® Plus 647 Anti-Human CD107a / LAMP1 Rabbit scFv Antibody (CL647-SF98266-2)

• Site-specific conjugation

CD107a / LAMP1 Recombinant Antibody for IF/ICC, FC (Intra)

Cat No. CL647-SF98266-2

Clone No.242116E8

Host / Type

Rabbit / scFv

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

CD107a, LAMP1, CD107 antigen-like family member A, CD107a/LAMP1, LAMP 1

• 

  IF Staining of HeLa using CL647-SF98266-2

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 Anti-Human CD107a / LAMP1 Rabbit scFv Antibody (CL647-SF98266-2, Clone: 242116E8 ) at dilution of 1:200.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of Jurkat using CL647-SF98266-2

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Human CD107a / LAMP1 Rabbit scFv Antibody (CL647-SF98266-2, Clone: 242116E8) (red) or 0.25 ug CoraLite® Plus 647 Rabbit scFv Isotype Control (CL647-SF98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-SF98266-2 targets CD107a / LAMP1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Type: Rabbit / scFv
• Class: Recombinant
• Type: Single-chain variable fragment antibody (scFv)
• Immunogen:

  CatNo: Eg2611

  Product name: Recombinant Human CD107a/LAMP1 protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 26-381 aa of NM_005561.4

  Sequence: ASAAMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENS

• Full Name: lysosomal-associated membrane protein 1
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: NM_005561.4
• Gene Symbol: LAMP1
• Gene ID (NCBI): 3916
• ENSEMBL Gene ID: ENSG00000185896
• RRID: AB_3748947
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Source: Anti-Human CD107a / LAMP1 scFv from clone 242116E8, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His tag at the C-terminus.
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin300, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).