Anti-Human CD107b / LAMP2 (H4B4)

• 

  IF Staining of U-251 using 65053-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using CD107b / LAMP2 antibody (65053-1-Ig, Clone: H4B4 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

• 

  IF Staining of MCF-7 using 65053-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CD107b / LAMP2 antibody (65053-1-Ig, Clone: H4B4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 65053-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107b / LAMP2 (65053-1-Ig, Clone:H4B4) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or Mouse IgG1 Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

CD107b / LAMP2 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. 65053-1-Ig

Clone No.H4B4

Publications(1)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

CD107b, LAMP2, LAMP-2, LAMP 2, H4B4

---

Tested Applications

• Positive IF/ICC detected in: MCF-7 cells, U-251 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.2 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

65053-1-Ig targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human adherent peripheral blood cells
• Full Name: lysosomal-associated membrane protein 2
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: BC002965
• Gene Symbol: LAMP2
• Gene ID (NCBI): 3920
• ENSEMBL Gene ID: ENSG00000005893
• RRID: AB_2881469
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P13473
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

Publications

Species	Application	Title
human	IF	J Biol Chem; Calmodulin enhances mTORC1 signaling by preventing TSC2-Rheb binding; Authors - Yuna Amemiya