Tested Applications
| Positive IF/ICC detected in | MCF-7 cells, U-251 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65053-1-Ig targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Human adherent peripheral blood cells Predict reactive species |
| Full Name | lysosomal-associated membrane protein 2 |
| Calculated Molecular Weight | 45 kDa |
| GenBank Accession Number | BC002965 |
| Gene Symbol | LAMP2 |
| Gene ID (NCBI) | 3920 |
| ENSEMBL Gene ID | ENSG00000005893 |
| RRID | AB_2881469 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P13473 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD107b / LAMP2 antibody 65053-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-Human CD107b/LAMP2 (H4B4) antibody (Cat# 65053-1-Ig) has 2 citations. These appear in Journal of Biological Chemistry and Biochemical Journal. The associated research fields include mTORC1 signaling regulation, calmodulin-mediated signaling, and G protein-coupled receptor pathways, all studied in human samples using immunofluorescence applications.
| Species | Application | Title |
|---|---|---|
Biochem J Substrate-specific regulation of the mTORC1 pathway by G protein-coupled receptors. |
Reviews
What customers say
All three reviewers gave this antibody a perfect 5-star rating for immunofluorescence. Users report clear, specific lysosomal staining with a typical punctate pattern and low background. One reviewer noted that methanol fixation yielded better results than PFA, which produced higher background. Tested dilutions ranged from 1:100 to 1:500 across Hap1 cells and mammalian cell lines. Overall, customers find it reliable and easy to use for lysosomal visualization by IF.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shivali (Verified Customer) (10-07-2025) | LAMP2 antibody gave clear, specific lysosomal staining by IF. Methanol fixation worked best, while PFA resulted in poor signal and higher background.
 |
Parijat (Verified Customer) (09-23-2025) | Works great for IF. I could see typical lysosomal punctuate pattern with this antibody.
|
Lianna (Verified Customer) (07-17-2025) | Looks good for immunofluorescence. Clean staining
|
