Tested Applications
| Positive IF/ICC detected in | MCF-7 cells, U-251 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
65053-1-Ig targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Human adherent peripheral blood cells Predict reactive species |
| Full Name | lysosomal-associated membrane protein 2 |
| Calculated Molecular Weight | 45 kDa |
| GenBank Accession Number | BC002965 |
| Gene Symbol | LAMP2 |
| Gene ID (NCBI) | 3920 |
| ENSEMBL Gene ID | ENSG00000005893 |
| RRID | AB_2881469 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P13473 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD107b / LAMP2 antibody 65053-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Shivali (Verified Customer) (10-07-2025) | LAMP2 antibody gave clear, specific lysosomal staining by IF. Methanol fixation worked best, while PFA resulted in poor signal and higher background.
 |
FH Parijat (Verified Customer) (09-23-2025) | Works great for IF. I could see typical lysosomal punctuate pattern with this antibody.
|
FH Lianna (Verified Customer) (07-17-2025) | Looks good for immunofluorescence. Clean staining
|
