Anti-Human CD107b / LAMP2 (H4B4), PBS Only

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• Anti-Human CD107b / LAMP2 (H4B4), PBS Only (65053-1-PBS)

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  IF Staining of U-251 using 65053-1-Ig (same clone as 65053-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using CD107b / LAMP2 antibody (65053-1-Ig, Clone: H4B4 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 65053-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 65053-1-Ig (same clone as 65053-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CD107b / LAMP2 antibody (65053-1-Ig, Clone: H4B4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 65053-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 65053-1-Ig (same clone as 65053-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107b / LAMP2 (65053-1-Ig, Clone:H4B4) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or Mouse IgG1 Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. This data was developed using the same antibody clone with 65053-1-PBS in a different storage buffer formulation.

CD107b / LAMP2 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. 65053-1-PBS

Clone No.H4B4

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

CD107b, LAMP2, CD107 antigen-like family member B, LAMP 2, LAMP-2

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65053-1-Ig

Product Information

65053-1-PBS targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Human adherent peripheral blood cells

• Full Name: lysosomal-associated membrane protein 2
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: BC002965
• Gene Symbol: LAMP2
• Gene ID (NCBI): 3920
• ENSEMBL Gene ID: ENSG00000005893
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P13473
• Storage Buffer: PBS Only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).