FITC Anti-Human CD107b (H4B4)

CD107b Monoclonal Antibody for IF,FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF,FC

Conjugate

FITC Fluorescent Dye

CloneNo.

H4B4

Cat no : FITC-65053

Synonyms

CD107b, LAMP 2, LAMP2, LAMPB, LGP110



Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

FITC-65053 targets CD107b in IF,FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Human adherent peripheral blood cells
Full Name lysosomal-associated membrane protein 2
Calculated molecular weight 45 kDa
GenBank accession numberBC002965
Gene symbol LAMP2
Gene ID (NCBI) 3920
Conjugate FITC Fluorescent Dye
Excitation/Emission maxima wavelengths494 nm/520 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

Protocols

Product Specific Protocols
IF protocol for FITC CD107b antibody FITC-65053Download protocol
FC protocol for FITC CD107b antibody FITC-65053Download protocol
Standard Protocols
Click here to view our Standard Protocols