CoraLite® Plus 647 Anti-Human CD107b / LAMP2 Mouse scFv Antibody

• Home
• Products
• Primary Antibodies
• CoraLite® Plus 647 Anti-Human CD107b / LAMP2 Mouse scFv Antibody (CL647-SF65566)

• Site-specific conjugation

CD107b / LAMP2 Recombinant Antibody for IF/ICC, FC (Intra)

Cat No. CL647-SF65566

Clone No.H4B4

Host / Type

Mouse / scFv

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

CD107b, LAMP2, CD107 antigen-like family member B, H4B4, LAMP 2

• 

  IF Staining of HeLa using CL647-SF65566

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 CD107b / LAMP2 antibody (CL647-SF65566, Clone: H4B4) at dilution of 1:200.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of Jurkat using CL647-SF65566

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Human CD107b / LAMP2 Mouse scFv Antibody (CL647-SF65566, Clone: H4B4) (red) or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-SF65566 targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Type: Mouse / scFv
• Class: Recombinant
• Type: Single-chain variable fragment antibody (scFv)
• Immunogen:

  Human adherent peripheral blood cells

• Full Name: lysosomal-associated membrane protein 2
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: BC002965
• Gene Symbol: LAMP2
• Gene ID (NCBI): 3920
• ENSEMBL Gene ID: ENSG00000005893
• RRID: AB_3748937
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Source: Anti-Human CD107b / LAMP2 scFv from clone H4B4, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His tag at the C-terminus.
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin 300, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).