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CD109 Polyclonal antibody

CD109 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 18715-1-AP

Synonyms

CD109, CD109 antigen, CD109 molecule, CPAMD7, p180, Platelet specific Gov antigen, r150, RP11 525G3.1

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using CD109 Polyclonal antibody (18715-1-AP)

    WB analysis of PC-3 using 18715-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18715-1-AP (CD109 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using CD109 Polyclonal antibody (18715-1-AP)

    IP experiment of PC-3 using 18715-1-AP

    IP result of anti-CD109 (IP:18715-1-AP, 4ug; Detection:18715-1-AP 1:600) with PC-3 cells lysate 5000ug.

Tested Applications

Positive WB detected inPC-3 cells
Positive IP detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18715-1-AP targets CD109 in WB, IP,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name CD109 molecule
Calculated Molecular Weight 162 kDa
GenBank Accession NumberBC152996
Gene Symbol CD109
Gene ID (NCBI) 135228
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD109, also named as CPAMD7, is a member of the alpha2-macroglobulin/complement superfamily. It is a glycosylphosphatidylinositol (GPI)-linked cell surface glycoprotein of approximately 170kd which is found on the cell surface of platelets, activated T-cells, and endothelial cells. CD109 binds to and negatively regulates signaling of transforming growth factor beta (TGF-beta). CD109 has been identified as part of the TGF-beta receptor system in human keratinocytes. Up regulation of CD109 expression has been observed in several different types of tumor.CD109 is a glycosyl-phosphatidylinositol-anchored glycoprotein that is a member of the a2-macroglobulin/C3, C4, C5 family of thioester-containing proteins. There're 2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form. The antibody can recognize all these 4 isoforms.

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

CD109 expression is upregulated in penile squamous cell carcinoma.

Authors - Fengyun Dong
View Article