FC experiment of human peripheral blood monocytes using 65160-1-Ig
1X10^6 human peripheral blood monocytes were surface stained with 0.5 ug Anti-Human CD116 (65160-1-Ig, Clone: 4H1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.5 ug isotype control and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.
1X10^6 human peripheral blood monocytes were surface stained with 0.5 ug Anti-Human CD116 (65160-1-Ig, Clone: 4H1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.5 ug isotype control and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
65160-1-Ig targets CD116 in FC applications and shows reactivity with Human samples.
FC experiment of human peripheral blood monocytes using 65160-1-Ig
1X10^6 human peripheral blood monocytes were surface stained with 0.5 ug Anti-Human CD116 (65160-1-Ig, Clone: 4H1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.5 ug isotype control and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD116 Monoclonal antibody
Catalog Number
65160-1-Ig
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
