Tested Applications
| Positive FC detected in | mouse bone marrow cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.4 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
APC-65054 targets CD117/c-Kit in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Mouse IL-3-dependent bone marrow mast cells Predict reactive species |
| Full Name | kit oncogene |
| GenBank Accession Number | BC052457 |
| Gene Symbol | CD117 |
| Gene ID (NCBI) | 16590 |
| RRID | AB_2882957 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
| Excitation Laser | Red Laser (633 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P05532 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for APC CD117/c-Kit antibody APC-65054 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
APC-65054 is an Anti-Mouse CD117/c-Kit (2B8) monoclonal antibody. It has 1 indexed citation in Advanced Science (Impact Factor 14.1), published in 2026. The study used flow cytometry to identify and sort spermatogonial stem cells marked by IL1R2, demonstrating their role in restoring spermatogenesis after interruption in mammals. The research falls within reproductive biology and stem cell biology.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) IL1R2 Marks and Sorts a Spermatogonial Stem Cell Population Critical for Restoring Spermatogenesis Upon Interruption in Mammals. |
