PE Anti-Human CD117 (104D2)

CD117 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1 kappa

Reactivity

Human, Bovine

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

104D2

Cat no : PE-65154

Synonyms

C Kit, CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit



Tested Applications

Positive FC detected inTF-1 cells

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 20 µl per 10^6 cells in a 100 µl suspension or 20 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

PE-65154 targets CD117 in FC applications and shows reactivity with Human, Bovine samples.

Tested Reactivity Human, Bovine
Host / Isotype Mouse / Mouse IgG1 kappa
Class Monoclonal
Type Antibody
Immunogen MOLM-1 megakaryocytic cells
Full Name v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Calculated molecular weight 976 aa, 110 kDa
GenBank accession numberBC071593
Gene symbol KIT
Gene ID (NCBI) 3815
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496, 565 nm/578 nm
Form Liquid
Purification Method The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

Protocols

Product Specific Protocols
FC protocol for PE CD117 antibody PE-65154Download protocol
Standard Protocols
Click here to view our Standard Protocols