Anti-Human CD11a (TS2/4) Rabbit IgG Recombinant Antibody, PBS Only

• 

  FC experiment of human PBMCs using 65518-1-RR (same clone as 65518-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-RR, Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65518-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65518-1-PBS

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-PBS Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65518-1-PBS

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-PBS, Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65518-1-RR (same clone as 65518-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-RR, Clone:TS2/4) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65518-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65518-1-PBS

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-PBS Clone:TS2/4) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.2 ug Anti-Human CD11a Mouse Monoclonal Antibody (Clone:TS2/4) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed.

• 

  FC experiment of human PBMCs using 65518-1-RR (same clone as 65518-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD11a Rabbit Recombinant Antibody (65518-1-RR, Clone:TS2/4) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or with 0.2 ug Anti-Human CD11a Mouse Monoclonal Antibody (Clone:TS2/4) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65518-1-PBS in a different storage buffer formulation.

CD11a Uni-rAb^® Recombinant Antibody for FC

Cat No. 65518-1-PBS

Clone No.TS2/4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD11 antigen-like family member A, Integrin alpha L, Integrin alpha-L, ITGAL, Leukocyte adhesion glycoprotein LFA-1 alpha chain

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65518-1-PBS targets CD11a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  HLA-DR CTL line

• Full Name: integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
• Calculated Molecular Weight: 129 kDa
• GenBank Accession Number: BC008777
• Gene Symbol: CD11a
• Gene ID (NCBI): 3683
• ENSEMBL Gene ID: ENSG00000005844
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P20701
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).