Filter: AllWBIHCIF-PIF/ICC

WB analysis of Ramos using 66256-1-Ig (same clone as 66256-1-PBS)
Ramos cells were subjected to SDS PAGE followed by western blot with 66256-1-Ig (CD11a/Integrin Alpha L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66256-1-Ig (CD11a/Integrin alpha-L antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66256-1-Ig (CD11a/Integrin alpha-L antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.
IF Staining of Jurkat using 66256-1-Ig (same clone as 66256-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

CD11a/Integrin Alpha L Monoclonal antibody, PBS Only

CD11a/Integrin Alpha L Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 66256-1-PBS

Clone No.1E3H12

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

CD11a, 1E3H12, CD11 antigen-like family member A, Integrin alpha L, Integrin alpha-L

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66256-1-PBS targets CD11a/Integrin Alpha L in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag7813 Product name: Recombinant human CD11a/Integrin alpha-L protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 25-350 aa of BC008777 Sequence: SYNLDVRGARSFSPPRAGRHFGYRVLQVGNGVIVGAPGEGNSTGSLYQCQSGTGHCLPVTLRGSNYTSKYLGMTLATDPTDGSILFAAVQFSTSYKTEFDFSDYVKRKDPDALLKHVKHMLLLTNTFGAINYVATEVFREELGARPDATKVLIIITDGEATDSGNIDAAKDIIRYIIGIGKHFQTKESQETLHKFASKPASEFVKILDTFEKLKDLFTELQKKIYVIEGTSKQDLTSFNMELSSSGISADLSRGHAVVGAVGAKDWAGGFLDLKADLQDDTFIGNEPLTPEVRAGYLGYTVTWLPSRQKTSLLASGAPRYQHMGRV Predict reactive species
Full Name	integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
Calculated Molecular Weight	129 kDa
GenBank Accession Number	BC008777
Gene Symbol	CD11a
Gene ID (NCBI)	3683
ENSEMBL Gene ID	ENSG00000005844
RRID	AB_2881644
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P20701
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).