CD11a/Integrin Alpha L Monoclonal antibody
CD11a/Integrin Alpha L Monoclonal Antibody for IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1E3H12
Cat no : 66256-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human tonsillitis tissue |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Product Information
66256-1-Ig targets CD11a/Integrin Alpha L in IHC, IF, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD11a/Integrin Alpha L fusion protein Ag7813 |
Full Name | integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) |
Calculated molecular weight | 129 kDa |
GenBank accession number | BC008777 |
Gene symbol | ITGAL |
Gene ID (NCBI) | 3683 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).
Protocols
Product Specific Protocols | |
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IHC protocol for CD11a/Integrin Alpha L antibody 66256-1-Ig | Download protocol |
IF protocol for CD11a/Integrin Alpha L antibody 66256-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |