Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PY5P5-FcA65582 targets CD11b in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Rheumatoid synovial cells and human monocytes Predict reactive species |
| Full Name | integrin, alpha M (complement component 3 receptor 3 subunit) |
| Calculated Molecular Weight | 1152 aa, 127 kDa |
| GenBank Accession Number | BC096346 |
| Gene Symbol | CD11b |
| Gene ID (NCBI) | 3684 |
| ENSEMBL Gene ID | ENSG00000169896 |
| RRID | AB_3749955 |
| Conjugate | PE-Cyanine5.5 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 692 nm |
| Excitation Laser | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P11215 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE-Cyanine5.5 CD11b antibody PY5P5-FcA65582 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
PY5P5-FcA65582 has 1 citation to date. The cited study was published in European Journal of Medical Research (IF 4.8) and investigated the mechanism of ultrasound-guided renal parenchymal injection of mesenchymal stem cells for treating chronic kidney disease, specifically examining how down-regulated SGK1 promotes M2 macrophage polarization. The antibody was applied in flow cytometry on human samples. The research falls within the fields of nephrology, immunology, and regenerative medicine.
| Species | Application | Title |
|---|---|---|
Eur J Med Res Mechanism of ultrasound-guided renal parenchymal injection of MSCs for the treatment of chronic kidney disease: down-regulated SGK1 promotes M2 macrophage polarization. |
