FcZero-rAb® CoraLite® Plus 405 Anti-Rat CD11b/c (OX42) Rabbit Recombinant Antibody

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• FcZero-rAb® CoraLite® Plus 405 Anti-Rat CD11b/c (OX42) Rabbit Recombinant Antibody (CL405-FcA65606)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD11b/c FcZero-rAb^® Recombinant Antibody for FC

Cat No. CL405-FcA65606 Copied!

Clone No. OX42

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Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

FC

Synonyms

Cd11b, integrin alpha M, Itgam, OX42

• 

  FC experiment of rat bone marrow cells using CL405-FcA65606

  1x10^6 rat bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 405 Anti-Rat CD11b/c (OX42) Rabbit RecAb (CL405-FcA65606, Clone: OX42) or 0.25 ug Rabbit IgG Isotype Control RecAb (CL405-FcA98136, Clone: 240953C9). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of rat bone marrow cells using CL405-FcA65606

  1x10^6 rat bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 405 Anti-Rat CD11b/c (OX42) Rabbit RecAb (CL405-FcA65606, Clone: OX42) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (CL405-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: rat bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL405-FcA65606 targets CD11b/c in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Rat peritoneal macrophages

• Full Name: integrin alpha M
• Calculated Molecular Weight: 127 kDa
• GenBank Accession Number: NM_012711
• Gene Symbol: CD11b
• Gene ID (NCBI): 25021
• RRID: AB_3747189
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Excitation Laser: Violet Laser (405 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9JI30
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b (integrin alpha M or CR3A) and CD11c (integrin alpha X) are members of the integrin alpha chain family and can form α/β heterodimers with CD18 (integrin β2). CD11b/CD18 is a receptor for the complement protein fragment C3bi, and is also a receptor for fibrinogen, factor X, and ICAM1 (PMID: 2971974; 15485828). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533). The OX-42 antibody recognizes a common epitope shared between CD11b and CD11c. It binds to C3bi receptor and is known to block C3bi binding (PMID: 3512425; 8449218).