Anti-Human CD11c (3.9) Mouse IgG2a Recombinant Antibody

• 

  FC experiment of human peripheral blood leukocyte using 65568-1-MR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD11c Mouse IgG2a Recombinant Antibody (65568-1-MR, Clone: 3.9) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocyte using 65568-1-MR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD11c Mouse IgG2a Recombinant Antibody (65568-1-MR, Clone: 3.9) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocyte using 65568-1-MR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD11c Mouse IgG2a Recombinant Antibody (65568-1-MR, Clone:3.9) (left) or 0.25 ug Anti-Human CD11c (65086-1-Ig, Clone: 3.9) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD11c Uni-rAb^TM Recombinant Antibody for FC

Cat No. 65568-1-MR

Clone No.3.9

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

Leu M5, ITGAX, Integrin alpha-X, Integrin alpha X, CD11 antigen-like family member C

---

Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65568-1-MR targets CD11c in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Rheumatoid synovial cells and human monocytes
• Full Name: integrin, alpha X (complement component 3 receptor 4 subunit)
• Calculated Molecular Weight: 1169 aa, 129 kDa
• GenBank Accession Number: BC038237
• Gene Symbol: CD11c
• Gene ID (NCBI): 3687
• ENSEMBL Gene ID: ENSG00000140678
• RRID: AB_3670322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).