Anti-Mouse CD11c (N418) Mouse IgG2a Recombinant Antibody, PBS Only

• 

  FC experiment of mouse splenocytes using 65602-1-MR (same clone as 65602-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD11c (N418) Mouse IgG2a Recombinant Antibody (65602-1-MR, Clone: N418) (right), or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (left) and FITC anti-mouse IgG2a Antibody. Cells well then stained with APC Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) (APC-65122, Clone: M5/114.15.2). Cells were not fixed. This data was developed using the same antibody clone with 65602-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 65602-1-MR (same clone as 65602-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD11c (N418) Mouse IgG2a Recombinant Antibody (65602-1-MR, Clone: N418) and FITC anti-Mouse IgG2a Antibody (right) or 0.25 ug Anti-Mouse CD11c (N418) (65130-1-Ig, Clone: N418) and FITC anti-Armenian Hamster IgG Antibody (left). Cells well then stained with APC Anti-Mouse MHC Class II (I-A/I-E). Cells were not fixed. This data was developed using the same antibody clone with 65602-1-PBS in a different storage buffer formulation.

CD11c Uni-rAb^® Recombinant Antibody for FC

Cat No. 65602-1-PBS

Clone No.N418

Host / Isotype

Mouse / IgG2a

Reactivity

mouse

Applications

FC

Synonyms

Itgax, Cr4, integrin alpha X, N418

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Product Information

65602-1-PBS targets CD11c in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Mouse spleen dendritic cells

• Full Name: integrin alpha X
• GenBank Accession Number: BC167225
• Gene Symbol: CD11c
• Gene ID (NCBI): 16411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9QXH4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).