FcZero-rAb® Biotin Anti-Human CD122/IL-2RB Rabbit Recombinant Antibody

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• FcZero-rAb® Biotin Anti-Human CD122/IL-2RB Rabbit Recombinant Antibody (Biotin-FcA98151)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD122/IL-2RB FcZero-rAb^® Recombinant Antibody for FC, ELISA

Cat No. Biotin-FcA98151

Clone No.241677H7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, ELISA

Synonyms

IL2RB, CD122, High affinity IL-2 receptor subunit beta, IL 2 receptor subunit beta, IL 2R subunit beta

• 

  FC experiment of human PBMCs using Biotin-FcA98151

  1x10^6 human PBMCs were surface stained with APC Anti-Human NCAM1/CD56, 0.25 ug Biotin Anti-Human CD122/IL-2RB Rabbit RecAb (Biotin-FcA98151, Clone:241677H7) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-FcA98151

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD122/IL-2RB Rabbit RecAb (Biotin-FcA98151, Clone: 241677H7) (red) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98151 targets CD122/IL-2RB in FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: interleukin 2 receptor, beta
• Calculated Molecular Weight: 551 aa, 61 kDa
• GenBank Accession Number: BC025691
• Gene Symbol: IL2RB
• Gene ID (NCBI): 3560
• ENSEMBL Gene ID: ENSG00000100385
• RRID: AB_3746943
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14784
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The interleukin-2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to the ability to bind interleukin-2. The low-affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. The beta subunit (IL-2RB, also known as CD122 and p75) is involved in receptor-mediated endocytosis and transduces the mitogenic signals of interleukin-2.