PE Anti-Human IL3RA/CD123 (HI12H7)

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  FC experiment of human PBMCs using PE-65256

  1X10^6 human PBMCs were surface co-stained with CL647 Anti-Human CD45RO and 5 ul PE Anti-Human CD123 (PE-65256, Clone:HI12H7), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

IL3RA/CD123 Monoclonal Antibody for FC

Cat No. PE-65256

Clone No.HI12H7

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Synonyms

IL3RA, IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-65256 targets IL3RA/CD123 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  N/A

• Full Name: interleukin 3 receptor, alpha (low affinity)
• Calculated Molecular Weight: 378 aa, 43 kDa
• GenBank Accession Number: BC035407
• Gene Symbol: CD123
• Gene ID (NCBI): 3563
• ENSEMBL Gene ID: ENSG00000185291
• RRID: AB_3085026
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P26951
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD123, also referred to as interleukin-3 receptor subunit alpha (IL-3Rα), belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). CD123 is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to CD123 and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.