Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-65093 targets IL-7Ra/CD127 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
IL-7Ra-IgG1 fusion protein Predict reactive species |
| Full Name | interleukin 7 receptor |
| GenBank Accession Number | BC089571 |
| Gene Symbol | CD127 |
| Gene ID (NCBI) | 16197 |
| RRID | AB_2883887 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Excitation Laser | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P16872 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.1% gelatin, pH 7.2. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD127, also known as IL-7R subunit alpha (IL-7Rα), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells (PMID: 8415665). IL-7R is a heterodimer composed of CD127 and IL-2Rγ (CD132), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15 (PMID: 8266077; 9862091). IL-7R plays critical roles in lymphocyte development and homeostasis (PMID: 26336149). CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP) (PMID: 10974032).
Publications
What published studies show
PE-65093 is the PE-conjugated form of Anti-Mouse IL-7Ra/CD127 (clone A7R34), catalog number 65093. It has a total of 1 citation. The citation appears in Journal of Inflammation Research (IF 4.6), published in 2023. The study investigated how sodium butyrate ameliorates type 2 diabetes-related sarcopenia via the IL-33-independent ILC2s/IL-13/STAT3 signaling pathway in mice, using flow cytometry to detect CD127 expression. The research fields encompass immunology, metabolic disease, and muscle biology.
| Species | Application | Title |
|---|---|---|
J Inflamm Res Sodium Butyrate Ameliorates Type 2 Diabetes-Related Sarcopenia Through IL-33-Independent ILC2s/IL-13/STAT3 Signaling Pathway |
