PE Anti-Mouse CD127 (IL-7Ra) (A7R34)

CD127 (IL-7Ra) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

PE Fluorescent Dye

Publications

1

CloneNo.

A7R34

Cat no : PE-65093

Synonyms

CD127, IL 7Ralpha, IL- 7Ralpha, Il7r, IL-7Ra, interleukin 7 receptor

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using PE-65093

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug APC-Anti-Mouse CD3 (APC-65077, clone 17A2) and 0.25 ug PE-Anti-Mouse CD127 (IL-7Ra) (PE-65093, clone A7R34). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using PE-65093

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug APC-Anti-Mouse CD3 (APC-65077, clone 17A2) and 0.25 ug PE-rat IgG2a isotype control. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

PE-65093 targets CD127 (IL-7Ra) in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-7Ra-IgG1 fusion protein
• Full Name: interleukin 7 receptor
• GenBank Accession Number: BC089571
• Gene Symbol: Il7r
• Gene ID (NCBI): 16197
• RRID: AB_2883887
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

CD127, also known as IL-7R subunit alpha (IL-7Rα), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells (PMID: 8415665). IL-7R is a heterodimer composed of CD127 and IL-2Rγ (CD132), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15 (PMID: 8266077; 9862091). IL-7R plays critical roles in lymphocyte development and homeostasis (PMID: 26336149). CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP) (PMID: 10974032).

Publications

Species	Application	Title
mouse	FC	J Inflamm Res; Sodium Butyrate Ameliorates Type 2 Diabetes-Related Sarcopenia Through IL-33-Independent ILC2s/IL-13/STAT3 Signaling Pathway; Authors - Yuan Cao