APC Anti-Human CD13 (WM15)

CD13 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1

Reactivity

Human, Non-human primates

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

WM15

Cat no : APC-65186

Synonyms

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN



Tested Applications

Positive FC detected inhuman peripheral blood granulocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

APC-65186 targets CD13 in FC applications and shows reactivity with Human, Non-human primates samples.

Tested Reactivity Human, Non-human primates
Host / Isotype Mouse / Mouse IgG1
Class Monoclonal
Type Antibody
Immunogen Human AML cells
Full Name alanyl (membrane) aminopeptidase
Calculated molecular weight 110 kDa
GenBank accession numberBC058928
Gene symbol ANPEP
Gene ID (NCBI) 290
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/660 nm
Form Liquid
Purification Method The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD13, also named as APN, ANPEP (aminopeptidase N) or PEPN, is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)

Protocols

Product Specific Protocols
FC protocol for APC CD13 antibody APC-65186Download protocol
Standard Protocols
Click here to view our Standard Protocols