APC Anti-Mouse CD134/OX40 (OX-86)

CD134/OX40 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

mouse

Applications

FC

Conjugate

APC Fluorescent Dye

Publications

1

CloneNo.

OX-86

Cat no : APC-65136

Synonyms

Tnfrsf4, CD134, Txgp1, OX-86, Ox40

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Validation Data Gallery

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  FC experiment of BALB/C mouse splenocytes using APC-65136

  1X10^6 unstimulated or anti-CD3/CD28 stimulated (2 days) mouse splenocytes were surface co-stained with FITC Plus Anti-Mouse CD3 (17A2) (FITC-65077, Clone: 17A2) and 0.2 ug APC Anti-Mouse CD134 (APC-65136, Clone: OX-86). Cells were not fixed.

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 treated mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

APC-65136 targets CD134/OX40 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant mouse OX-40-CD4 chimeric protein
• Full Name: tumor necrosis factor receptor superfamily, member 4
• GenBank Accession Number: BC065782
• Gene Symbol: CD134
• Gene ID (NCBI): 22163
• RRID: AB_2883017
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).

Publications

Species	Application	Title
mouse	FC	Biomed Res; Blockade of CD80/CD86-CD28 co-stimulation augments the inhibitory function of peptide antigen-specific regulatory T cells; Authors - Yui Maehara