CD134/OX40 Polyclonal antibody, PBS Only

• 

  WB analysis using 20006-1-AP (same clone as 20006-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20006-1-AP (CD134/OX40 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20006-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 20006-1-AP (same clone as 20006-1-PBS)

  1X10^6 day 3 PHA treated human PBMCs were surface stained with 0.2 ug Anti-Human CD134/OX40 (20006-1-AP) and CoraLite®750-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or unstained (blue). Unstimulated human PBMCs were surface stained with 0.2ug Anti-Human CD134/OX40 (20006-1-AP) and CoraLite®750-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (black dashed). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 20006-1-PBS in a different storage buffer formulation.

CD134/OX40 Polyclonal Antibody for WB, FC, Indirect ELISA

Cat No. 20006-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, Indirect ELISA

Synonyms

TNFRSF4, ACT35, ACT35 antigen, CD134, OX40

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Product Information

20006-1-PBS targets CD134/OX40 in WB, FC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tumor necrosis factor receptor superfamily, member 4
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 50-55 kDa, 40 kDa
• GenBank Accession Number: NM_003327
• Gene Symbol: CD134
• Gene ID (NCBI): 7293
• RRID: AB_10733472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43489
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD134 (OX40) is a member of the TNFR-superfamily of receptors. Predominantly expressed on activated T cells, CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule. CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmunity (PMID: 26215166). CD134 can interact with TRAF2, TRAF3, and TRAF5.