CD134/OX40 Polyclonal antibody

CD134/OX40 Polyclonal Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20006-1-AP

Synonyms

TNFRSF4, TXGP1L, ACT35, ACT35 antigen, CD134, OX40, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor

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Validation Data Gallery

• 

  WB analysis using 20006-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20006-1-AP (CD134/OX40 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of human PBMCs using 20006-1-AP

  1X10^6 day 3 PHA treated human PBMCs were surface stained with 0.2 ug Anti-Human CD134/OX40 (20006-1-AP) and CoraLite®750-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or unstained (blue). Unstimulated human PBMCs were surface stained with 0.2ug Anti-Human CD134/OX40 (20006-1-AP) and CoraLite®750-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (black dashed). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive WB detected in: MJ cells
• Positive FC detected in: PHA treated human PBMCs

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

20006-1-AP targets CD134/OX40 in WB, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tumor necrosis factor receptor superfamily, member 4
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 50-55 kDa, 40 kDa
• GenBank Accession Number: NM_003327
• Gene Symbol: CD134
• Gene ID (NCBI): 7293
• RRID: AB_10733472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD134 (OX40) is a member of the TNFR-superfamily of receptors. Predominantly expressed on activated T cells, CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule. CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmunity (PMID: 26215166). CD134 can interact with TRAF2, TRAF3, and TRAF5.

Publications

Species	Application	Title
human	WB	Front Immunol; A Novel Immune-Related Gene Signature to Identify the Tumor Microenvironment and Prognose Disease Among Patients With Oral Squamous Cell Carcinoma Patients Using ssGSEA: A Bioinformatics and Biological Validation Study; Authors - Yun Chen
human	WB	Dis Markers; Development of a Costimulatory Molecule Signature to Predict Prognosis, Immune Landscape, and Response to Immune Therapy for Hepatocellular Carcinoma; Authors - Yongjie Zhou

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