CD14 Polyclonal antibody

CD14 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

36

Reviews (1)

Cat no : 17000-1-AP

Synonyms

CD14, CD14 molecule

---

Validation Data Gallery

• 

  WB analysis of HL-60 using 17000-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17000-1-AP (CD14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 17000-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 17000-1-AP (CD14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 17000-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 17000-1-AP (CD14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 17000-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17000-1-AP (CD14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with CD14 antibody included
• 

  IHC staining of human hepatocirrhosis using 17000-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17000-1-AP (CD14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with CD14 antibody included
• 

  IHC staining of human tonsillitis using 17000-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17000-1-AP (CD14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with CD14 antibody included
• 

  IHC staining of human tonsillitis using 17000-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17000-1-AP (CD14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with CD14 antibody included
• 

  IF Staining of RAW 264.7 using 17000-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed RAW 264.7 cells using 17000-1-AP (CD14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of RAW 264.7 using 17000-1-AP

  1X10^6 RAW 264.7 cells were stained with 0.2ug CD14 antibody (17000-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Tested Applications

• Positive WB detected in: HL-60 cells, RAW 264.7 cells, human spleen tissue
• Positive IHC detected in: human hepatocirrhosis tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: RAW 264.7 cells
• Positive FC detected in: RAW 264.7 cells

Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.

Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 7 publications below
• IHC: See 14 publications below
• IF: See 14 publications below
• FC: See 2 publications below

Product Information

17000-1-AP targets CD14 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD14 fusion protein Ag10693
• Full Name: CD14 molecule
• Calculated molecular weight: 375 aa, 40 kDa
• Observed molecular weight: 50-55 kDa
• GenBank accession number: BC010507
• Gene symbol: CD14
• Gene ID (NCBI): 929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Publications

Species	Application	Title
mouse	IF	Biomed Pharmacother; Short-interval exposure to ambient fine particulate matter (PM2.5) exacerbates the susceptibility of pulmonary damage in setting of lung ischemia-reperfusion injury in rodent: Pharmacomodulation of melatonin.; Authors - Fan-Yen Lee
rat	IHC,IF	Am J Transl Res; Adipose-derived mesenchymal stem cell-derived exosomes markedly protected the brain against sepsis syndrome induced injury in rat.; Authors - Chia-Lo Chang
human	IF	Br J Sports Med; Chronic inflammation is a feature of Achilles tendinopathy and rupture.; Authors - Stephanie Georgina Dakin
human	WB	Virchows Arch; Immune cell landscape in therapy-naïve squamous cell and adenocarcinomas of the lung.; Authors - Luka Brcic
human	WB	Acta Physiol (Oxf); Acute physical exercise and long-term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells.; Authors - A Zietzer
rat	IF	J Neuropathol Exp Neurol; Combined Adipose-Derived Mesenchymal Stem Cells and Low-Energy Extracorporeal Shock Wave Therapy Protect the Brain From Brain Death-Induced Injury in Rat.; Authors - Kuan-Hung Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue @ 1:200 with a Tris-EDTA antigen retrieval.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Prostate

Related Products

CD14 Monoclonal antibody

60253-1-Ig

TLR4 Polyclonal antibody

19811-1-AP

TNF Alpha Monoclonal antibody

60291-1-Ig

CD68 Polyclonal antibody

25747-1-AP

CD68 Monoclonal antibody

66231-1-Ig

LBP Polyclonal antibody

11836-1-AP