CD14 Polyclonal antibody

CD14 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17000-1-AP

Synonyms

CD14, CD14 molecule



Tested Applications

Positive WB detected inHL-60 cells, RAW 264.7 cells, human spleen tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells
Positive FC detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17000-1-AP targets CD14 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, pig, RAT
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD14 fusion protein Ag10693
Full Name CD14 molecule
Calculated molecular weight 375 aa, 40 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC010507
Gene symbol CD14
Gene ID (NCBI) 929
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein. CD14 is preferentially expressed on monocytes and macrophages. CD14 acts as a co-receptor (along with TLR4 and MD-2) for bacterial liposaccharides (LPS). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Product Specific Protocols
WB protocol for CD14 antibody 17000-1-APDownload protocol
IHC protocol for CD14 antibody 17000-1-APDownload protocol
IF protocol for CD14 antibody 17000-1-APDownload protocol
FC protocol for CD14 antibody 17000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Biomed Pharmacother

Short-interval exposure to ambient fine particulate matter (PM2.5) exacerbates the susceptibility of pulmonary damage in setting of lung ischemia-reperfusion injury in rodent: Pharmacomodulation of melatonin.

Authors - Fan-Yen Lee
ratIHC,IF

Am J Transl Res

Adipose-derived mesenchymal stem cell-derived exosomes markedly protected the brain against sepsis syndrome induced injury in rat.

Authors - Chia-Lo Chang
humanIF

Br J Sports Med

Chronic inflammation is a feature of Achilles tendinopathy and rupture.

Authors - Stephanie Georgina Dakin
humanWB

Virchows Arch

Immune cell landscape in therapy-naïve squamous cell and adenocarcinomas of the lung.

Authors - Luka Brcic
humanWB

Acta Physiol (Oxf)

Acute physical exercise and long-term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells.

Authors - A Zietzer
ratIF

J Neuropathol Exp Neurol

Combined Adipose-Derived Mesenchymal Stem Cells and Low-Energy Extracorporeal Shock Wave Therapy Protect the Brain From Brain Death-Induced Injury in Rat.

Authors - Kuan-Hung Chen