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CD14 Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 17000-1-AP

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Con: 35 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
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Images See all

CD14 Antibody WB HL-60 cells 17000-1-AP

HL-60 cells were subjected to SDS PAGE followed by western blot with 17000-1-AP( CD14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

CD14 Antibody IHC human liver tissue 17000-1-AP

Immunohistochemistry of paraffin-embedded human liver tissue slide using 17000-1-AP( CD14 Antibody) at dilution of 1:200 (under 10x lens).

CD14 Antibody IHC human liver tissue 17000-1-AP

Immunohistochemistry of paraffin-embedded human liver tissue slide using 17000-1-AP( CD14 Antibody) at dilution of 1:200 (under 40x lens).

CD14 Antibody IF RAW 264.7 cells 17000-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed RAW 264.7 cells using 17000-1-AP(CD14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

CD14 Antibody FC RAW 264.7 cells 17000-1-AP

1X10^6 RAW 264.7 cells were stained with 0.2ug CD14 antibody (17000-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, RAT, swine
Positive WB detected in:
HL-60 cells, RAW 264.7 cells, human spleen tissue
Positive IHC detected in:
human liver tissue, human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
RAW 264.7 cells
Positive FC detected in:
RAW 264.7 cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:100-1:400
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CD14 fusion protein Ag10693
Full name:
CD14 molecule
Calculated molecular weight:
375aa,40 kDa
Observed molecular weight:
50-55 kDa
GenBank accession number:
BC010507
Gene ID (NCBI):
929
Gene symbol
CD14
Synonyms
CD14, CD14 molecule

Background

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein. CD14 is preferentially expressed on monocytes and macrophages. CD14 acts as a co-receptor (along with TLR4 and MD-2) for bacterial liposaccharides (LPS). It plays a major role in the inflammatory response of monocytes to LPS.


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