CD14 Polyclonal antibody
CD14 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 17000-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HL-60 cells, RAW 264.7 cells, human spleen tissue |
Positive IHC detected in | human hepatocirrhosis tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | RAW 264.7 cells |
Positive FC detected in | RAW 264.7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 4 publications below |
IHC | See 10 publications below |
IF | See 10 publications below |
FC | See 1 publications below |
Product Information
17000-1-AP targets CD14 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, RAT, swine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CD14 fusion protein Ag10693 |
Full Name | CD14 molecule |
Calculated molecular weight | 375 aa, 40 kDa |
Observed molecular weight | 50-55 kDa |
GenBank accession number | BC010507 |
Gene symbol | CD14 |
Gene ID (NCBI) | 929 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein. CD14 is preferentially expressed on monocytes and macrophages. CD14 acts as a co-receptor (along with TLR4 and MD-2) for bacterial liposaccharides (LPS). It plays a major role in the inflammatory response of monocytes to LPS.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CD14 antibody 17000-1-AP | Download protocol |
IHC protocol for CD14 antibody 17000-1-AP | Download protocol |
IF protocol for CD14 antibody 17000-1-AP | Download protocol |
FC protocol for CD14 antibody 17000-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biomed Pharmacother Short-interval exposure to ambient fine particulate matter (PM2.5) exacerbates the susceptibility of pulmonary damage in setting of lung ischemia-reperfusion injury in rodent: Pharmacomodulation of melatonin. | ||
Am J Transl Res Adipose-derived mesenchymal stem cell-derived exosomes markedly protected the brain against sepsis syndrome induced injury in rat. | ||
Virchows Arch Immune cell landscape in therapy-naïve squamous cell and adenocarcinomas of the lung. | ||
Acta Physiol (Oxf) Acute physical exercise and long-term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells. | ||
J Neuropathol Exp Neurol Combined Adipose-Derived Mesenchymal Stem Cells and Low-Energy Extracorporeal Shock Wave Therapy Protect the Brain From Brain Death-Induced Injury in Rat. |