CoraLite® Plus 488-conjugated CD141/Thrombomodulin Recombinant monoclonal antibody

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• CoraLite® Plus 488-conjugated CD141/Thrombomodulin Recombinant monoclonal antibody (CL488-84412-3)

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  IF Staining of bEnd.3 using CL488-84412-3

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CoraLite® Plus 488 CD141/Thrombomodulin antibody (CL488-84412-3, Clone: 241730D4 ) at dilution of 1:200.

CD141/Thrombomodulin Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-84412-3

Clone No.241730D4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC

Synonyms

CD141, Fetomodulin, Thbd, Thrombomodulin, TM

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Tested Applications

• Positive IF/ICC detected in: bEnd.3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84412-3 targets CD141/Thrombomodulin in IF/ICC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1139

  Product name: Recombinant Mouse Thrombomodulin/CD141 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 17-517 aa of NM_009378.3

  Sequence: LSALAKLQPTGSQCVEHECFALFQGPATFLDASQACQRLQGHLMTVRSSVAADVISLLLSQSSMDLGPWIGLQLPQGCDDPVHLGPLRGFQWVTGDNHTSYSRWARPNDQTAPLCGPLCVTVSTATEAAPGEPAWEEKPCETETQGFLCEFYFTASCRPLTVNTRDPEAAHISSTYNTPFGVSGADFQTLPVGSSAAVEPLGLELVCRAPPGTSEGHWAWEATGAWNCSVENGGCEYLCNRSTNEPRCLCPRDMDLQADGRSCARPVVQSCNELCEHFCVSNAEVPGSYSCMCETGYQLAADGHRCEDVDDCKQGPNPCPQLCVNTKGGFECFCYDGYELVDGECVELLDPCFGSNCEFQCQPVSPTDYRCICAPGFAPKPDEPHKCEMFCNETSCPADCDPNSPTVCECPEGFILDEGSVCTDIDECSQGECFTSECRNFPGSYECICGPDTALAGQISKDCDPIPVREDTKEEEGSGEPPVSPTPGSPTGPPSARPVHS

• Full Name: thrombomodulin
• Calculated Molecular Weight: 62kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: NM_009378.3
• Gene Symbol: Thbd
• Gene ID (NCBI): 21824
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15306
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).