FITC Plus Anti-Mouse CD147 Rabbit Recombinant Antibody

• 

  FC experiment of mouse thymocytes using FITC-98229

  1x10^6 mouse thymocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD147 Rabbit RecAb (FITC-98229, Clone:241852B4),or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9).Cells were not fixed.

• 

  FC experiment of mouse thymocytes using FITC-98229

  1x10^6 mouse thymocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD147 Rabbit RecAb (FITC-98229, Clone:241852B4)(red),or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9)(blue). Cells were not fixed.

CD147 Uni-rAb^TM Recombinant Antibody for FC

Cat No. FITC-98229

Clone No.241852B4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

241852B4, Basic immunoglobulin superfamily, Basigin, Bsg, HT7 antigen

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Tested Applications

• Positive FC detected in: mouse thymocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-98229 targets CD147 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: basigin
• Calculated Molecular Weight: 42kDa
• GenBank Accession Number: NM_009768.2
• Gene Symbol: CD147
• Gene ID (NCBI): 12215
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P18572-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211).