CoraLite® Plus 488 Anti-Mouse CD40L/CD154 (MR1)

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  FC experiment of BALB/c mouse CD4+ T cells using CL488-65129

  1X10^6 unstimulated or Cell Stimulation Cocktail stimulated (overnight) BALB/c mouse CD4+ T cells were surface co-stained with PE Anti-Mouse CD4 (GK1.5) and 0.5 ug CoraLite® Plus 488 Anti-Mouse CD154 (CL488-65129, Clone: MR1). Cells were not fixed.

CD40L/CD154 Monoclonal Antibody for FC

Cat No. CL488-65129

Clone No.MR1

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

FC

Synonyms

CD154, CD40 ligand, Cd40l, Cd40lg, gp39, HIGM1, IGM, IMD3, Ly 62, Ly62, MR1, T BAM, Tnfsf5, TRAP

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Tested Applications

• Positive FC detected in: Cell Stimulation Cocktail stimulated mouse CD4+ T cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65129 targets CD40L/CD154 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD40 ligand
• GenBank Accession Number: BC119225
• Gene Symbol: CD154
• Gene ID (NCBI): 21947
• RRID: AB_2919266
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.