Anti-Mouse CD16 / CD32 (2.4G2)

CD16 / CD32 Monoclonal Antibody for FC (Intra)

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

Publications

1

CloneNo.

2.4G2

Cat no : 65080-1-Ig

Synonyms

CD32, 2.4G2, Fcr 2, FcgRII, Fcgr2b

---

Validation Data Gallery

• 

  FC experiment of mouse splenocytes using 65080-1-Ig

  1x10^6 mouse splenocytes were stained with 0.5 ug Anti-Mouse CD16 / CD32 (65080-1-Ig, Clone: 2.4G2) and FITC anti-rat IgG2b Antibody at dilution 1:100, or 0.5 ug Control Antibody and FITC anti-rat IgG2b Antibody at dilution 1:100. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were not fixed.

---

Tested Applications

• Positive FC (Intra) detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

65080-1-Ig targets CD16 / CD32 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: Fc receptor, IgG, low affinity IIb
• GenBank Accession Number: BC038070
• Gene Symbol: CD16/32
• Gene ID (NCBI): 14130
• RRID: AB_2918383
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. The 2.4G2 antibody reacts with the extracellular domains of mouse CD16 (FcRIII) and CD32 (FcRII) (PMID: 90108; 8406898). Rat anti mouse CD16/CD32 antibody, clone 2.4G2 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors by preincubating the cells with the purified antibody at 1 ug per million cells in 100 uL volume for 5-10 minutes prior to immunostaining.

Publications

Species	Application	Title
mouse	FC	Front Pharmacol; In-situ formed thermosensitive hydrogel amplifies statin-mediated immune checkpoint blockade for coordinated tumor chemo-immunotherapy; Authors - Zefan Liu