Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65080-1-Ig targets CD16/CD32 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | Fc receptor, IgG, low affinity IIb |
| GenBank Accession Number | BC038070 |
| Gene Symbol | CD16/32 |
| Gene ID (NCBI) | 14130 |
| RRID | AB_2918383 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P08101 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. The 2.4G2 antibody reacts with the extracellular domains of mouse CD16 (FcRIII) and CD32 (FcRII) (PMID: 90108; 8406898). Rat anti mouse CD16/CD32 antibody, clone 2.4G2 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors by preincubating the cells with the purified antibody at 1 ug per million cells in 100 uL volume for 5-10 minutes prior to immunostaining.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD16/CD32 antibody 65080-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Mouse CD16/CD32 (2.4G2), catalog 65080-1-Ig, has 5 citations. They appear in Frontiers in Pharmacology, Frontiers in Bioscience, Journal of Biochemical and Molecular Toxicology, International Immunology, and Breast Cancer Research and Treatment. The research spans tumor chemo-immunotherapy and immune checkpoint blockade, LPS-induced acute lung injury, PD-L1-driven immune evasion in NSCLC, allergic rhinitis, and bispecific antibody antitumor therapy—primarily using flow cytometry in mouse models.
| Species | Application | Title |
|---|---|---|
Front Pharmacol In-situ formed thermosensitive hydrogel amplifies statin-mediated immune checkpoint blockade for coordinated tumor chemo-immunotherapy | ||
Front Biosci (Landmark Ed) Macrophage Notch1 Participates in LPS-Induced Acute Lung Injury via Regulating CCR5 Expression in Mice | ||
J Biochem Mol Toxicol Toxicological Implications of m(6)A Dysregulation: SH3PXD2A-AS1-Driven PD-L1 Overexpression Promotes Immune Evasion in NSCLC. | ||
Int Immunol Role of Group 2 innate lymphoid cells in intranasal sensitization-induced allergic rhinitis in mice. | ||
Breast Cancer Res Treat Enhanced antitumor efficacy of bispecific antibody blocking PD-L1 and LAG-3 with doxorubicin: mechanism and safety evaluation |
Reviews
What customers say
The single review for this Anti-mouse CD16/CD32 antibody (rated 4/5) highlights its strong specificity and consistent blocking efficiency in Fc receptor inhibition assays. The reviewer used it for immunofluorescence, flow cytometry, and cell culture on RAW264.7 and DC2.4 mouse immune cells, finding it highly reliable for these applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ishan (Verified Customer) (10-03-2025) | The Anti-mouse CD16/CD32 antibody from ProteiTech shows strong specificity and consistent blocking efficiency, making it highly reliable for Fc receptor inhibition assays in mouse immune cells.
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