Anti-Mouse CD16 / CD32 (2.4G2)

CD16 / CD32 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b









Cat no : 65080-1-Ig


AI528646, CD16 / CD32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1

Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65080-1-Ig targets CD16 / CD32 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name Fc receptor, IgG, low affinity IIb
GenBank accession numberBC038070
Gene symbol Fcgr2b
Gene ID (NCBI) 14130
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide, pH 7.2.
Storage ConditionsStore at 2-8°C. 20ul sizes contain 0.1% BSA.

Background Information

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. The 2.4G2 antibody reacts with the extracellular domains of mouse CD16 (FcRIII) and CD32 (FcRII) (PMID: 90108; 8406898). Rat anti mouse CD16/CD32 antibody, clone 2.4G2 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors.


Product Specific Protocols
FC protocol for CD16 / CD32 antibody 65080-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols