|Positive FC detected in||mouse splenocytes|
|This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.|
|Sample-dependent, check data in validation data gallery|
65080-1-Ig targets CD16 / CD32 in FC applications and shows reactivity with Mouse samples.
|Host / Isotype||Rat / IgG2b|
|Full Name||Fc receptor, IgG, low affinity IIb|
|GenBank accession number||BC038070|
|Gene ID (NCBI)||14130|
|Purification Method||Affinity purification|
|Storage Buffer||Phosphate based buffer with 0.09% sodium azide, pH 7.2.|
|Storage Conditions||Store at 2-8°C.|
CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. The 2.4G2 antibody reacts with the extracellular domains of mouse CD16 (FcRIII) and CD32 (FcRII) (PMID: 90108; 8406898). Rat anti mouse CD16/CD32 antibody, clone 2.4G2 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors.