CoraLite® Plus 647 Anti-Mouse CD16 / CD32 (2.4G2)

CD16 / CD32 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2.4G2

Cat no : CL647-65080

Synonyms

AI528646, CD16 / CD32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1

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Validation Data Gallery

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  FC experiment of C57BL/6 mouse splenocytes using CL647-65080

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with FITC Plus Anti-Mouse CD3 (17A2) and 0.125 ug CoraLite® Plus 647 Anti-Mouse CD16 / CD32 (CL647-65080, Clone:2.4G2) or 0.125 ug CoraLite® Plus 647 Rat IgG2b Isotype Control (LTF-2). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65080 targets CD16 / CD32 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: Fc receptor, IgG, low affinity IIb
• GenBank Accession Number: BC038070
• Gene Symbol: Fcgr2b
• Gene ID (NCBI): 14130
• RRID: AB_2883668
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. The 2.4G2 antibody reacts with the extracellular domains of mouse CD16 (FcRIII) and CD32 (FcRII) (PMID: 90108; 8406898).