Mouse CD163 Polyclonal antibody, PBS Only

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• Mouse CD163 Polyclonal antibody, PBS Only (31024-1-PBS)

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  WB analysis using 31024-1-AP (same clone as 31024-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31024-1-AP (CD163 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 31024-1-AP (same clone as 31024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31024-1-AP (CD163 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 31024-1-AP (same clone as 31024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31024-1-AP (CD163 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 31024-1-AP (same clone as 31024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31024-1-AP (CD163 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 31024-1-AP (same clone as 31024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31024-1-AP (CD163 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 31024-1-AP (same clone as 31024-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CD163 antibody (31024-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 31024-1-AP (same clone as 31024-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CD163 antibody (31024-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

CD163 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 31024-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

sCD163, M130

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Product Information

31024-1-PBS targets CD163 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: CD163 antigen
• Calculated Molecular Weight: 121 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: NM_053094.2
• Gene Symbol: Cd163
• Gene ID (NCBI): 93671
• RRID: AB_3669819
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q2VLH6-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).