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WB analysis using 33399-1-AP
mouse spleen tissue was subjected to SDS-PAGE followed by western blot with 33399-1-AP (CD169 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 33399-1-AP
Rat spleen tissue was subjected to SDS-PAGE followed by western blot with 33399-1-AP (CD169 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse spleen using 33399-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 33399-1-AP (CD169 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 33399-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 33399-1-AP (CD169 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 33399-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 33399-1-AP (CD169 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 33399-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 33399-1-AP (CD169 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD169 Polyclonal antibody

Name: CD169 Polyclonal antibody
Brand: Proteintech
SKU: 33399-1-AP

CD169 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 33399-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Sa, SER, Sheep erythrocyte receptor, Sialic acid-binding Ig-like lectin 1, Sialoadhesin

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse spleen tissue, rat spleen tissue
Positive IHC detected in	mouse spleen tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

33399-1-AP targets CD169 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity	mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Eg5002 Product name: Recombinant Mouse Siglec1/CD169 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 20-510 aa of NP_035556 Sequence: TWGVSSPKNVQGLSGSCLLIPCIFSYPADVPVSNGITAIWYYDYSGKRQVVIHSGDPKLVDKRFRGRAELMGNMDHKVCNLLLKDLKPEDSGTYNFRFEISDSNRWLDVKGTTVTVTTDPSPPTITIPEELREGMERNFNCSTPYLCLQEKQVSLQWRGQDPTHSVTSSFQSLEPTGVYHQTTLHMALSWQDHGRTLLCQFSLGAHSSRKEVYLQVPHAPKGVEILLSSSGRNILPGDPVTLTCRVNSSYPAVSAVQWARDGVNLGVTGHVLRLFSAAWNDSGAYTCQATNDMGSLVSSPLSLHVFMAEVKMNPAGPVLENETVTLLCSTPKEAPQELRYSWYKNHILLEDAHASTLHLPAVTRADTGFYFCEVQNAQGSERSSPLSVVVRYPPLTPDLTTFLETQAGLVGILHCSVVSEPLATVVLSHGGLTLASNSGENDFNPRFRISSAPNSLRLEIRDLQPADSGEYTCLAVNSLGNSTSSLDFYAN Predict reactive species
Full Name	sialic acid binding Ig-like lectin 1, sialoadhesin
Calculated Molecular Weight	183 kDa
Observed Molecular Weight	200 kDa
GenBank Accession Number	NP_035556
Gene Symbol	Siglec1
Gene ID (NCBI)	20612
RRID	AB_3742960
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity Purification
UNIPROT ID	Q62230-1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD169, also known as sheep erythrocyte receptor (SER), Sialoadhesin (Sn) or Siglec-1, is a sialic acid-binding immunoglobulin-like lectin that belongs to the SIGLEC family of the Ig superfamily (PMID: 3783087; 11133773). CD169 is a single-pass transmembrane protein containing a long extracellular domain, a transmembrane region, and a short cytoplasmic tail (PMID: 11133773). It is primarily expressed on the surface of specific macrophage subsets and its precursor monocytes, as well as on some DCs (PMID: 33408860). CD169 binds to sialic acid-containing glycoconjugates, particularly those with α(2,3)-linkages. It is involved in immune regulation, pathogen recognition, and cell-cell interactions.