Tested Applications
| Positive IHC detected in | human tonsillitis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
Product Information
55427-1-AP targets CD169 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | sialic acid binding Ig-like lectin 1, sialoadhesin |
| Calculated Molecular Weight | 183 kDa |
| GenBank Accession Number | NM_023068 |
| Gene Symbol | CD169 |
| Gene ID (NCBI) | 6614 |
| RRID | AB_2881327 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BZZ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD169, also known as sheep erythrocyte receptor (SER), Sialoadhesin (Sn) or Siglec-1, is a sialic acid-binding immunoglobulin-like lectin that belongs to the SIGLEC family of the Ig superfamily (PMID: 3783087; 11133773). CD169 is a single-pass transmembrane protein containing a long extracellular domain, a transmembrane region, and a short cytoplasmic tail (PMID: 11133773). It is primarily expressed on the surface of specific macrophage subsets and its precursor monocytes, as well as on some DCs (PMID: 33408860). CD169 binds to sialic acid-containing glycoconjugates, particularly those with α(2,3)-linkages. It is involved in immune regulation, pathogen recognition, and cell-cell interactions.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD169 antibody 55427-1-AP | Download protocol |
| IHC protocol for CD169 antibody 55427-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
BMC Vet Res Curcumin is a promising inhibitor of genotype 2 porcine reproductive and respiratory syndrome virus infection. | ||
J Cancer Res Clin Oncol Dysregulation of SIGLEC1 in non-small cell lung cancer: prognostic implications and immunomodulatory role-a multicenter cohort study | ||
Discov Oncol SIGLEC1 has the potential to be an immune-related prognostic indicator in colon adenocarcinoma: a study based on transcriptomic data and Mendelian randomization analysis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Emma (Verified Customer) (02-22-2022) | Works well by IF on FFPE prostate tissue. (Tris-EDTA antigen retrieval).
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