CD169 Polyclonal antibody

CD169 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 55427-1-AP

Synonyms

CD169, Sialoadhesin, SIGLEC 1, SIGLEC1, SN

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Validation Data Gallery

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  IHC staining of human liver using 55427-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55427-1-AP (CD169 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 55427-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55427-1-AP (CD169 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human tonsillitis using 55427-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55427-1-AP (CD169 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human tonsillitis using 55427-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55427-1-AP (CD169 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human tonsillitis using 55427-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD169 antibody (55427-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 55427-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD169 antibody (55427-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human liver tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

55427-1-AP targets CD169 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: sialic acid binding Ig-like lectin 1, sialoadhesin
• Calculated Molecular Weight: 183 kDa
• GenBank Accession Number: NM_023068
• Gene Symbol: CD169
• Gene ID (NCBI): 6614
• RRID: AB_2881327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
monkey	WB	BMC Vet Res; Curcumin is a promising inhibitor of genotype 2 porcine reproductive and respiratory syndrome virus infection.; Authors - Taofeng Du

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emma (Verified Customer) (02-22-2022)

Works well by IF on FFPE prostate tissue. (Tris-EDTA antigen retrieval).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:25
• Cell Tissue Type: Prostate