CD169 Polyclonal antibody, PBS Only

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  IHC staining of human liver using 55427-1-AP (same clone as 55427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55427-1-AP (CD169 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 55427-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 55427-1-AP (same clone as 55427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55427-1-AP (CD169 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 55427-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 55427-1-AP (same clone as 55427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55427-1-AP (CD169 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55427-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 55427-1-AP (same clone as 55427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55427-1-AP (CD169 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55427-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 55427-1-AP (same clone as 55427-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD169 antibody (55427-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55427-1-PBS in a different storage buffer formulation.

CD169 Polyclonal Antibody for IHC, IF-P, Indirect ELISA

Cat No. 55427-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF-P, Indirect ELISA

Synonyms

SIGLEC1, Sialoadhesin, Sialoadhesin/CD169, SIGLEC 1, SN

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Product Information

55427-1-PBS targets CD169 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: sialic acid binding Ig-like lectin 1, sialoadhesin
• Calculated Molecular Weight: 183 kDa
• GenBank Accession Number: NM_023068
• Gene Symbol: CD169
• Gene ID (NCBI): 6614
• RRID: AB_2881327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BZZ2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD169, also known as sheep erythrocyte receptor (SER), Sialoadhesin (Sn) or Siglec-1, is a sialic acid-binding immunoglobulin-like lectin that belongs to the SIGLEC family of the Ig superfamily (PMID: 3783087; 11133773). CD169 is a single-pass transmembrane protein containing a long extracellular domain, a transmembrane region, and a short cytoplasmic tail (PMID: 11133773). It is primarily expressed on the surface of specific macrophage subsets and its precursor monocytes, as well as on some DCs (PMID: 33408860). CD169 binds to sialic acid-containing glycoconjugates, particularly those with α(2,3)-linkages. It is involved in immune regulation, pathogen recognition, and cell-cell interactions.