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Anti-Human CD169 Rabbit Recombinant Antibody, PBS Only (98644-1-PBS)

Anti-Human CD169 Rabbit Recombinant Antibody, PBS Only

Name: Anti-Human CD169 Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98644-1-PBS-1MG

CD169 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98644-1-PBS

Clone No.251732D3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

SIGLEC1, Sialoadhesin, Sialoadhesin/CD169, SIGLEC 1, SN

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Filter: AllFC

FC experiment of human PBMCs using 98644-1-RR (same clone as 98644-1-PBS)
1x10^6 unstimulated or IFN alpha treated human PBMCs were surface stained with 0.25 ug Anti-Human CD169 Rabbit RecAb (98644-1-RR, Clone:251732D3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98644-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 unstimulated or IFN alpha treated human PBMCs were surface stained with 0.25 ug Anti-Human CD169 Rabbit RecAb (98644-1-RR, Clone:251732D3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98644-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 98644-1-RR (same clone as 98644-1-PBS)
1x10^6 IFN alpha treated human PBMCs were surface stained with 0.25 ug Anti-Human CD169 Rabbit RecAb (98644-1-RR, Clone:251732D3) (red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 unstimulated human PBMCs were surface stained with 0.25 ug Anti-Human CD169 Rabbit RecAb (98644-1-RR, Clone:251732D3)(black) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98644-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 IFN alpha treated human PBMCs were surface stained with 0.25 ug Anti-Human CD169 Rabbit RecAb (98644-1-RR, Clone:251732D3) (red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 unstimulated human PBMCs were surface stained with 0.25 ug Anti-Human CD169 Rabbit RecAb (98644-1-RR, Clone:251732D3)(black) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98644-1-PBS in a different storage buffer formulation.

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

98644-1-PBS targets CD169 in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	DNA Predict reactive species
Full Name	sialic acid binding Ig-like lectin 1, sialoadhesin
Calculated Molecular Weight	183 kDa
GenBank Accession Number	NM_023068
Gene Symbol	CD169
Gene ID (NCBI)	6614
RRID	AB_3746241
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9BZZ2
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD169, also known as sheep erythrocyte receptor (SER), Sialoadhesin (Sn) or Siglec-1, is a sialic acid-binding immunoglobulin-like lectin that belongs to the SIGLEC family of the Ig superfamily (PMID: 3783087; 11133773). CD169 is a single-pass transmembrane protein containing a long extracellular domain, a transmembrane region, and a short cytoplasmic tail (PMID: 11133773). It is primarily expressed on the surface of specific macrophage subsets and its precursor monocytes, as well as on some DCs (PMID: 33408860). CD169 binds to sialic acid-containing glycoconjugates, particularly those with α(2,3)-linkages. It is involved in immune regulation, pathogen recognition, and cell-cell interactions.