Anti-Human CD169 Rabbit Recombinant Antibody

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• Anti-Human CD169 Rabbit Recombinant Antibody (98644-1-RR)

CD169 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98644-1-RR

Clone No.251732D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

SIGLEC1, Sialoadhesin, Sialoadhesin/CD169, SIGLEC 1, SN

• 

  FC experiment of human PBMCs using 98644-1-RR

  1x10^6 unstimulated or IFN alpha treated human PBMCs were surface stained with 0.25 ug Anti-Human CD169 Rabbit RecAb (98644-1-RR, Clone:251732D3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using 98644-1-RR

  1x10^6 IFN alpha treated human PBMCs were surface stained with 0.25 ug Anti-Human CD169 Rabbit RecAb (98644-1-RR, Clone:251732D3) (red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 unstimulated human PBMCs were surface stained with 0.25 ug Anti-Human CD169 Rabbit RecAb (98644-1-RR, Clone:251732D3)(black) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: IFN alpha treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98644-1-RR targets CD169 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  DNA

• Full Name: sialic acid binding Ig-like lectin 1, sialoadhesin
• Calculated Molecular Weight: 183 kDa
• GenBank Accession Number: NM_023068
• Gene Symbol: CD169
• Gene ID (NCBI): 6614
• RRID: AB_3746241
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BZZ2
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD169, also known as sheep erythrocyte receptor (SER), Sialoadhesin (Sn) or Siglec-1, is a sialic acid-binding immunoglobulin-like lectin that belongs to the SIGLEC family of the Ig superfamily (PMID: 3783087; 11133773). CD169 is a single-pass transmembrane protein containing a long extracellular domain, a transmembrane region, and a short cytoplasmic tail (PMID: 11133773). It is primarily expressed on the surface of specific macrophage subsets and its precursor monocytes, as well as on some DCs (PMID: 33408860). CD169 binds to sialic acid-containing glycoconjugates, particularly those with α(2,3)-linkages. It is involved in immune regulation, pathogen recognition, and cell-cell interactions.