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Anti-Human CD16a Rabbit Recombinant Antibody, PBS Only (98293-1-PBS)

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FC experiment of human peripheral blood leukocytes using 98293-1-RR (same clone as 98293-1-PBS)
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16a Rabbit RecAb (98293-1-RR, Clone:242426G11) , or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98293-1-PBS in a different storage buffer formulation.
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1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16a Rabbit RecAb (98293-1-RR, Clone:242426G11) , or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98293-1-PBS in a different storage buffer formulation.
FC experiment of human peripheral blood leukocytes using 98293-1-RR (same clone as 98293-1-PBS)
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16a Rabbit RecAb (98293-1-RR, Clone:242426G11) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98293-1-PBS in a different storage buffer formulation.
×
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16a Rabbit RecAb (98293-1-RR, Clone:242426G11) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98293-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 98293-1-RR
Biolayer interferometry (BLl) kinetic assays of 98293-1-RR against Human CD16a were performed. The affinity constant is 14.3 nM.
98293-1-RR specifically recognizes human CD16a
Biolayer interferometry (BLl) kinetic assays of 98293-1-RR against Human CD16a 176F (A), CD16a 176V (B), CD16b NA1 (C) and CD16b NA2 (D) were performed. Affinity constants against two CD16a allotypes are 25.9 nM and 18.3 nM. No binding signal against CD16b allotypes was detected.

Anti-Human CD16a Rabbit Recombinant Antibody, PBS Only

CD16a Uni-rAb^® Recombinant Antibody for FC

Cat No. 98293-1-PBS

Clone No.242426G11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

CD16, 242426G11, CD16a antigen, CD16-II, Fc gamma RIII

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

98293-1-PBS targets CD16a in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg2488 Product name: recombinant human CD16a protein Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 10007017 of Sequence: Predict reactive species
Full Name	Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Calculated Molecular Weight	29 kDa
Gene Symbol	CD16a
Gene ID (NCBI)	2214
ENSEMBL Gene ID	ENSG00000203747
RRID	AB_3745959
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B. This antibody is specific for CD16a and does not recognize CD16b.