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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human CD16a Rabbit Recombinant Antibody (98293-1-RR)

    FC experiment of human peripheral blood leukocytes using 98293-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16a Rabbit RecAb (98293-1-RR, Clone:242426G11) , or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human CD16a Rabbit Recombinant Antibody (98293-1-RR)

    FC experiment of human peripheral blood leukocytes using 98293-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16a Rabbit RecAb (98293-1-RR, Clone:242426G11) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98293-1-RR

    Affinity and Kinetic Characterization of 98293-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98293-1-RR against Human CD16a were performed. The affinity constant is 14.3 nM.

Anti-Human CD16a Rabbit Recombinant Antibody

CD16a Uni-rAbTM Recombinant Antibody for FC
Cat No. 98293-1-RR
Clone No.242426G11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD16, 242426G11, Fc gamma RIII alpha, Fc gamma RIII, CD16-II

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive FC detected inhuman peripheral blood leukocytes

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98293-1-RR targets CD16a in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein Predict reactive species
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Gene Symbol CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B.

Protocols

Product Specific Protocols
FC protocol for CD16a antibody 98293-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols