Anti-Human CD178 (Fas ligand) (NOK-1) Mouse IgG2a Recombinant Antibody

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  FC experiment of Transfected HEK-293T using 65598-1-MR

  1x10^6 HEK-293T cells or CD178 transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human CD178 (Fas ligand) (NOK-1) Mouse IgG2a RecAb (65598-1-MR, Clone: NOK-1) and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed.

• 

  FC experiment of Transfected HEK-293T using 65598-1-MR

  1x10^6 HEK-293T cells or CD178 transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human CD178 (Fas ligand) (NOK-1) Mouse IgG2a RecAb (65598-1-MR, Clone: NOK-1) and PE-conjugated Goat Anti-Mouse IgG (red), or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were not fixed.

CD178 (Fas ligand) Uni-rAb^® Recombinant Antibody for FC

Cat No. 65598-1-MR

Clone No.NOK-1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

CD178, Fas ligand, FASLG, CD95-L, CD95L

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Tested Applications

• Positive FC detected in: Transfected HEK-293T cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65598-1-MR targets CD178 (Fas ligand) in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  L5178Y mouse T lymphoma cells expressing recombinant human FasL

• Full Name: Fas ligand (TNF superfamily, member 6)
• Calculated Molecular Weight: 281 aa, 31 kDa
• GenBank Accession Number: BC017502
• Gene Symbol: Fas Ligand
• Gene ID (NCBI): 356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P48023
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD178, also known as Fas ligand, is a type II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. It is expressed on NK cells, cytotoxic T lymphocytes and activated CD4+ Th1 cells. CD178 transduces apoptotic signal into cells by binding to FAS/CD95. It is involved as a death factor in the regulation of activation-induced cell death, establishment of immune privilege and tumor cell survival.