Mouse CD18 Recombinant monoclonal antibody, PBS Only (Detector)

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• Mouse CD18 Recombinant monoclonal antibody, PBS Only (Detector) (87481-1-PBS)

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  WB analysis using 87481-1-RR (same clone as 87481-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87481-1-RR (ITB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87481-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP03055-2

  The mean CD18 concentration was determined to be 3,214.8 pg/mL in RAW 264.7 cell extract based on a 1.2 mg/mL extract load and 5,220.1 pg/mL in J774A.1 cell extract based on a 1.2 mg/mL extract load.

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  Sandwich ELISA standard curve of MP03055-2

  Sandwich ELISA standard curve of MP03055-2, Mouse CD18 Recombinant Matched Antibody Pair - PBS only. 87481-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg10459. 87481-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-1250 pg/mL.

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  Affinity of 87481-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87481-1-RR against Mouse CD18 were performed. The affinity constant is 5.27 nM.

CD18 Uni-rAb^® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA

Cat No. 87481-1-PBS

Clone No.252753H7

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, Sandwich ELISA, Indirect ELISA

Synonyms

Itgb2, integrin beta 2, LAD, LCAMB, Lfa1

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Product Information

87481-1-PBS targets CD18 as part of a matched antibody pair:

MP03055-2: 87481-3-PBS capture and 87481-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10459

  Product name: Recombinant mouse ITB2 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-Twin-Strep-Tag

  Domain: 10011456 of

  Sequence: \

• Full Name: integrin beta 2
• Observed Molecular Weight: 85-90 kDa
• Gene Symbol: CD18
• Gene ID (NCBI): 16414
• RRID: AB_3745572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11835
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).