CD18 Recombinant monoclonal antibody

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• CD18 Recombinant monoclonal antibody (87481-2-RR)

• 

  WB analysis using 87481-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87481-2-RR (ITB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 87481-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87481-2-RR (CD18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 87481-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87481-2-RR (CD18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity of 87481-2-RR

  Biolayer interferometry (BLl) kinetic assays of 87481-2-RR against Mouse CD18 were performed. The affinity constant is 14.2 nM.

CD18 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 87481-2-RR

Clone No.252753A4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

Itgb2, integrin beta 2, LAD, LCAMB, Lfa1

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Tested Applications

• Positive WB detected in: A20 cells, RAW 264.7 cells, mouse spleen tissue, mouse thymus tissue
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87481-2-RR targets CD18 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10459

  Product name: Recombinant mouse ITB2 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-Twin-Strep-Tag

  Domain: 10011456 of

  Sequence: \

• Full Name: integrin beta 2
• Observed Molecular Weight: 85-90 kDa
• Gene Symbol: CD18
• Gene ID (NCBI): 16414
• RRID: AB_3745573
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during the immune response, and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).