CoraLite®594 Anti-Mouse CD18 (M18/2) Rat Recombinant Antibody

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• CoraLite®594 Anti-Mouse CD18 (M18/2) Rat Recombinant Antibody (CL594-65671)

CD18 Uni-rAb^® Recombinant Antibody for FC

Cat No. CL594-65671 Copied!

Clone No. M18/2

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Host / Isotype

Rat / IgG2a

Reactivity

mouse

Applications

FC

Synonyms

Itgb2, integrin beta 2, LAD, LCAMB, Lfa1

• 

  FC experiment of mouse thymocytes using CL594-65671

  1x10^6 mouse thymocytes were surface stained with 0.25 ug CoraLite®594 Anti-Mouse CD18 (M18/2) Rat IgG2a RecAb (CL594-65671, Clone: M18/2), or CoraLite®594 Rat IgG2a Isotype Control (2A3) (CL594-65209, Clone: 2A3). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of mouse thymocytes using CL594-65671

  1x10^6 mouse thymocytes were surface stained with 0.25 ug CoraLite®594 Anti-Mouse CD18 (M18/2) Rat IgG2a RecAb (CL594-65671, Clone: M18/2) (red), or CoraLite®594 Rat IgG2a Isotype Control (2A3) (CL594-65209, Clone: 2A3) (blue). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: mouse thymocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65671 targets CD18 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  N/A

• Full Name: integrin beta 2
• GenBank Accession Number: BC145644
• Gene Symbol: CD18
• Gene ID (NCBI): 16414
• RRID: AB_3748147
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Excitation Laser: Yellow-Green Laser (561 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11835
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).