FITC Anti-Mouse CD18 (M18/2)

CD18 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

FITC Fluorescent Dye

CloneNo.

M18/2

Cat no : FITC-65178

Synonyms

2E6, AI528527, Cd18, integrin beta 2, Itgb2, LAD, LCAMB, Lfa1, MF17

---

Validation Data Gallery

• 

  FC experiment of mouse splenocytes using FITC-65178

  1X10^6 mouse splenocytes were surface stained with 0.5 ug FITC Anti-Mouse CD18 (FITC-65178, Clone: M18/2) (red), or 0.5 ug Control Antibody. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using FITC-65178

  1X10^6 mouse splenocytes were surface stained with 0.5 ug FITC Anti-Mouse CD18 (FITC-65178, Clone: M18/2) (red), or 0.5 ug Control Antibody. Cells were not fixed.

---

Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65178 targets CD18 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse CTL glycoprotein
• Full Name: integrin beta 2
• GenBank Accession Number: BC145644
• Gene Symbol: Itgb2
• Gene ID (NCBI): 16414
• RRID: AB_2883816
• Conjugate: FITC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 494 nm / 520 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).