Anti-Mouse CD19 (1D3) Rat IgG2a Recombinant Antibody, PBS Only

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  FC experiment of mouse splenocytes using 65661-1-TR (same clone as 65661-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD19 (1D3) Rat IgG2a RecAb (65661-1-TR, Clone:1D3) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3), and FITC anti-Rat IgG2a antibody. Cells were then stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed. This data was developed using the same antibody clone with 65661-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 65661-1-TR (same clone as 65661-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD19 (1D3) Rat IgG2a RecAb (65661-1-TR, Clone: 1D3) (red) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed. This data was developed using the same antibody clone with 65661-1-PBS in a different storage buffer formulation.

CD19 Uni-rAb^® Recombinant Antibody for FC

Cat No. 65661-1-PBS

Clone No.1D3

Host / Isotype

Rat / IgG2a

Reactivity

mouse

Applications

FC

Synonyms

CD19 antigen

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Product Information

65661-1-PBS targets CD19 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  N/A

• Full Name: CD19 antigen
• GenBank Accession Number: BC156767
• Gene Symbol: Cd19
• Gene ID (NCBI): 12478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P25918
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).