Biotin Anti-Mouse CD19 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using Biotin-98287

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Biotin Anti-Mouse CD19 Rabbit RecAb (Biotin-98287, Clone: 242301C6) and CoraLite®488-conjugated streptavidin, or unstained . Cells were co-stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using Biotin-98287

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Biotin Anti-Mouse CD19 Rabbit RecAb (Biotin-98287, Clone:242301C6) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were not fixed.

CD19 Uni-rAb^TM Recombinant Antibody for FC

Cat No. Biotin-98287

Clone No.242301C6

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

242301C6, CD19 antigen

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-98287 targets CD19 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD19 antigen
• Calculated Molecular Weight: 60 kDa
• GenBank Accession Number: NM_009844.2
• Gene Symbol: Cd19
• Gene ID (NCBI): 12478
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P25918
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).