Tested Applications
| Positive FC detected in | pig PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-98714-2 targets CD19 in FC applications and shows reactivity with pig samples.
| Tested Reactivity | pig |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg7132 Product name: Recombinant pig CD19 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 23-304 aa of XM_013995590.1 Sequence: RPQEPYLVEAQEGGNAVLPCLEGPSEGPPEQLAWFRGSQSTPFLELSLGLPGLGIHVGPLGTLKEPQGTLLFIFNVSDQMGGFYLCQQGPPLDQSWQPGWTVNVKGSGELFRWNASDLNDPSCDLGARSSEGRRSSSSHPTRSKLYVWAKNQAKVLHTDLTCPPPNSTVNQSNSHDLTVAPGSTLSLSCGSSRASLVRGPISWIHVRPKKHVKLLSLNLTEDAQLREMWVMGSLRGKAVLLLPEATAQDADTYHCNHGNVTTQMRLKVTARSVWHWLLETGG Predict reactive species |
| Full Name | CD19 molecule |
| Calculated Molecular Weight | 63 kDa |
| GenBank Accession Number | XM_013995590.1 |
| Gene Symbol | CD19 |
| Gene ID (NCBI) | 397669 |
| RRID | AB_3748884 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Excitation Laser | Red Laser (633 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | A0A8D0M7A3 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD19 is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).
