FITC Plus Anti-Mouse CD19 (1D3) Rat IgG2a Recombinant Antibody

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  FC experiment of mouse splenocytes using FITC-65661

  1x10^6 mouse splenocytes were surface stained with APC Anti-Mouse CD3, and 0.25 ug FITC Plus Anti-Mouse CD19 (1D3) Rat IgG2a RecAb (FITC-65661, Clone: 1D3) or FITC Plus Rat IgG2a Isotype Control (2A3) (FITC-65209, Clone: 2A3). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using FITC-65661

  1x10^6 mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD19 (1D3) Rat IgG2a RecAb (FITC-65661, Clone: 1D3) (red) or FITC Plus Rat IgG2a Isotype Control (2A3) (FITC-65209, Clone: 2A3) (blue). Cells were not fixed.

CD19 Uni-rAb^® Recombinant Antibody for FC

Cat No. FITC-65661

Clone No.1D3

Host / Isotype

Rat / IgG2a

Reactivity

mouse

Applications

FC

Synonyms

1D3, B-lymphocyte antigen CD19, CD19 antigen

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65661 targets CD19 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  N/A

• Full Name: CD19 antigen
• GenBank Accession Number: BC156767
• Gene Symbol: Cd19
• Gene ID (NCBI): 12478
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P25918
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).