Anti-Human CD19 Rabbit scFv Antibody, PBS Only

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• Anti-Human CD19 Rabbit scFv Antibody, PBS Only (SF98560-1-PBS)

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  FC experiment of human PBMCs using SF98560-1-RR (same clone as SF98560-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug CD19 Recombinant Antibody (SF98560-1-RR, Clone: 250499A2) or 0.25 ug Rabbit scFv Isotype Control (SF98136-1-RR, Clone: 240953C9), and PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone:251107A1). Cells were then stained withCoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with SF98560-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using SF98560-1-RR (same clone as SF98560-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug CD19 Recombinant Antibody (SF98560-1-RR, Clone: 250499A2) (red) or 0.25 ug Rabbit scFv Isotype Control (SF98136-1-RR, Clone: 240953C9) (blue), and PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with SF98560-1-PBS in a different storage buffer formulation.

CD19 Recombinant Antibody for FC, Indirect ELISA

Cat No. SF98560-1-PBS

Clone No.250499A2

Host / Type

Rabbit / scFv

Reactivity

human

Applications

FC, Indirect ELISA

Synonyms

B lymphocyte antigen CD19, B4, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, CD19 molecule

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Product Information

SF98560-1-PBS targets CD19 in FC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Type: Rabbit / scFv
• Class: Recombinant
• Type: Single-chain variable fragment antibody (scFv)
• Immunogen:

  CatNo: Eg3335

  Product name: Recombinant Human CD19 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 20-291 aa of BC006338

  Sequence: PEEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWK

• Full Name: CD19 molecule
• Calculated Molecular Weight: 556 aa, 61 kDa
• GenBank Accession Number: BC006338
• Gene Symbol: CD19
• Gene ID (NCBI): 930
• ENSEMBL Gene ID: ENSG00000177455
• RRID: AB_3750164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).