Anti-Human CD1a Rabbit Recombinant Antibody

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• Anti-Human CD1a Rabbit Recombinant Antibody (98643-1-RR)

CD1a Uni-rAb^® Recombinant Antibody for FC

Cat No. 98643-1-RR

Clone No.251904D12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD1, CD1a molecule, FCB6, HTA1, hTa1 thymocyte antigen

• 

  FC experiment of MOLT-4 using 98643-1-RR

  1x10^6 MOLT-4 cells were surface stained with 0.25 ug Anti-Human CD1a Rabbit RecAb (98643-1-RR, Clone: 251904D12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human monocyte-derived mature dendritic cells using 98643-1-RR

  1x10^6 human monocyte-derived mature dendritic cells were surface stained with 0.25 ug Anti-Human CD1a Rabbit RecAb (98643-1-RR, Clone: 251904D12) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 98643-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98643-1-RR against Human CD1a were performed. The affinity constant is 11.8 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human monocyte-derived mature dendritic cells, MOLT-4 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98643-1-RR targets CD1a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  DNA

• Full Name: CD1a molecule
• Calculated Molecular Weight: 327 aa, 37 kDa
• GenBank Accession Number: BC031645
• Gene Symbol: CD1a
• Gene ID (NCBI): 909
• RRID: AB_3746240
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P06126
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD1a is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. During protein synthesis and maturation, CD1 family members bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface.