Atlantic Blue™ Anti-Human CD1c (L161)

• 

  FC experiment of human PBMCs using AB-65176

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 750 Anti-Human CD19 and 5 ul Atlantic Blue™ Anti-Human CD1c (L161) (AB-65176) or Atlantic Blue™ Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using AB-65176

  1x10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD1c (L161) (AB-65176) or Atlantic Blue™ Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

CD1c Monoclonal Antibody for FC

Cat No. AB-65176

Clone No.L161

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Synonyms

BDCA1, CD1, CD1c molecule, L161, R7

---

Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

AB-65176 targets CD1c in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  human thymocytes

• Full Name: CD1c molecule
• GenBank Accession Number: BC126465
• Gene Symbol: CD1C
• Gene ID (NCBI): 911
• RRID: AB_3672345
• Conjugate: Atlantic Blue™ Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 404 nm / 458 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P29017
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD1c molecule (CD1c, also known as R7, CD1, CD1A, and BDCA1) is a member of the CD1 family of transmembrane glycoproteins, which is synthesized in the endoplasmic reticulum and expressed on the monocytes, marginal zone B cells in lymph nodes as well as peripheral blood (PMID: 23468110). The CD1 proteins belong to the family of major histocompatibility complex (MHC) class I-like proteins that present lipid-based antigens to T cells (PMID: 15032598; 23127489). CD1c shows interaction with both αβ or γδ T cells and mediates T cell responses against mycobacterium tuberculosis (PMID: 10727456; 10786796).