FcZero-rAb® Biotin Anti-Mouse CD1d (1B1) Rabbit Recombinant Antibody

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• FcZero-rAb® Biotin Anti-Mouse CD1d (1B1) Rabbit Recombinant Antibody (Biotin-FcA65652)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD1d FcZero-rAb^® Recombinant Antibody for FC, ELISA

Cat No. Biotin-FcA65652 Copied!

Clone No. 1B1

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Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC, ELISA

Synonyms

1B1, AI747460, CD1.1, Cd1d1, CD1d1 antigen

• 

  FC experiment of mouse splenocytes using Biotin-FcA65652

  1x10^6 mouse splenocytes were surface stained with APC Anti-Mouse CD45R (B220) (RA3-6B2), and 0.25 ug Biotin Anti-Mouse CD1d (1B1) Rabbit IgG RecAb (Biotin-FcA65652, Clone:1B1) or 0.25 ug Rabbit IgG Isotype Control RecAb (Biotin-FcA98136, Clone: 240953C9), and PE-conjugated streptavidin. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA65652 targets CD1d in FC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  N/A

• Full Name: CD1d1 antigen
• Gene Symbol: CD1d
• Gene ID (NCBI): 12479
• RRID: AB_3746846
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11609
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD1d is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on NKT cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines.